Fig. 2: BET protein inhibition reduces MGMT expression in GBM. | Cell Death & Disease

Fig. 2: BET protein inhibition reduces MGMT expression in GBM.

From: BET protein inhibition sensitizes glioblastoma cells to temozolomide treatment by attenuating MGMT expression

Fig. 2: BET protein inhibition reduces MGMT expression in GBM.The alternative text for this image may have been generated using AI.

A qRT-PCR analysis of relative MGMT gene expression at 6, 12, 24 and 48 h after treatment with [100 μM] TMZ and JQ1 at the following concentrations: [250 nM] for LN-340, [100 nM] for T98G, and [1000 nM] for the sphere lines LN-2683GS and LN-4372GS. Each data point represents an independent experiment. Data were normalized to the respective DMSO treatment for each time point (baseline). Adjusted p-values (p) were determined by Dunnett’s multiple comparisons test following two-way ANOVA using Geisser-Greenhouse correction for variance heterogeneity and including interaction between time and treatment. Error bars are SD. *(p ≤ 0.05), **(P ≤ 0.01), ***(P ≤ 0.001). B Protein expression analysis of MGMT, β-Actin and α-Tubulin by western blot, 24, 48, 72 and 120 h after treatment. One of three representative biological replicates is shown. The quantification of three replicates is shown in Supplemental Fig. S2B. Full length western blots are available in Supplemental Material.

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