Fig. 4: JQ1 modulates repair of TMZ-induced DNA damage.
A Mean γ-H2AX integrated intensity analysis was performed on LN-340 cells using immunofluorescence (IF). Cells were pretreated with JQ1 [0.250 µM] for 5 days. On day 5, cells were treated with O6BG [10 µM] together with TMZ [100 µM]. Two additional TMZ treatments were given every 6 h, for a total of 3 TMZ treatments on day 5. End-point was set at 48 h after TMZ treatments. Data represent the mean from three independent biological experiments. The adjusted p-values were provided by Dunnett T3 multiple comparisons tests following one-way ANOVA. *(p ≤ 0.05), **(p ≤ 0.01), ***(P ≤ 0.001). Error bars are SD. B Cells of GBM line LN-229MGMTind_C12 were treated according to the same schedule as in (A), but at a lower JQ1 concentration [100 nM]. The experiments were performed in absence and presence of doxycycline [100 ng/ml] (Dox), respectively. Dox treatment induces ectopic expression of MGMT under the control of the Tet-ON promoter. Scale bar 100 μm.
