Fig. 5: AKAP8L upregulates SCD1 expression via mRNA stability.
From: AKAP8L enhances the stemness and chemoresistance of gastric cancer cells by stabilizing SCD1 mRNA
A qPCR assay showed the expression of lipid metabolism-related genes. B Western blot analysis showed the expression of SCD1 in AKAP8L or AKAP8L shRNA transfected GC cells. C BGC-823/Oxa or MKN-45/Oxa cells were transiently transfected with control or AKAP8L plasmid for 48 h and then treated with ActD for 0, 2, 4, 6, 8 h. The SCD1 mRNA level was determined using qPCR. The GAPDH mRNA level was used as a negative control. D RIP assay using total cell lysates of BGC-823/Oxa or MKN-45/Oxa cells was used to assess the interaction between AKAP8L and SCD1 mRNA. Enrichment of SCD1 mRNA in the AKAP8L-containing immunoprecipitated particles was measured using qPCR and normalized to input. The values represent the mean ± SD of three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001 independent Student’s t-test).
