Fig. 2: DDX17 silencing suppresses the migration and invasion of CRC cells in vitro and in vivo. | Cell Death & Disease

Fig. 2: DDX17 silencing suppresses the migration and invasion of CRC cells in vitro and in vivo.

From: DDX17 induces epithelial-mesenchymal transition and metastasis through the miR-149-3p/CYBRD1 pathway in colorectal cancer

Fig. 2: DDX17 silencing suppresses the migration and invasion of CRC cells in vitro and in vivo.

A, B RT‒qPCR (A) and western blot (B) analysis verified the downregulation of DDX17 mediated by two specific shRNAs targeting DDX17 in SW620 and LoVo cells. C Microscopic observations were recorded at 0, 24, 48, and 72 h after scratching the surface of a confluent layer of the indicated SW620 and LoVo cells. D The effects of DDX17 on cell migration and invasion were examined by transwell assays in SW620 and LoVo cells. E The numbers of adhesive SW620 and LoVo cells on the Matrigel were recorded after 30, 60, 90, and 120 min. F DDX17-deficient and control SW620 cells were injected into the spleens of nude mice. Representative bioluminescent and H&E staining images of the isolated liver tissues were obtained, and the light emissions were quantified. n.s. no significance, **P < 0.01, ***P < 0.001.

Back to article page