Fig. 5: USA300 induces pyroptosis, autophagy and apoptosis in mPEOs.
From: Use of mouse primary epidermal organoids for USA300 infection modeling and drug screening
A Immunofluorescence staining analysis of Staphylococcus aureus biomarkers and Loricrin, KRT10, Involucrin, and KRT14 in mPEOs at 0, 2, 4, 8, and 24 h after USA300 infection. White arrows represent the location of USA300, and red arrows represent the colocalization of USA300 with Loricrin, KRT10, Involucrin or KRT14. (Scale bars, 200 μm). B Immunofluorescence staining analysis of the Staphylococcus aureus biomarker and Cleaved-caspase-1 (p10) in mPEOs 0, 2, 4, 8, and 24 h after USA300 infection (scale bars, 200 μm). C Immunofluorescence staining analysis of Staphylococcus aureus biomarkers and LC3-II in mPEOs after USA300 infection at 0, 2, 4, 8, and 24 h (scale bars, 200 μm). D Immunofluorescence staining analysis of Staphylococcus aureus biomarkers and Cleaved-caspase-3 in mPEOs after USA300 infection at 0, 2, 4, 8, and 24 h (scale bars, 200 μm). E IL-1β production was determined using ELISA at 0, 2, 4, 8, and 24 h after mPEOs were infected with USA300.
