Fig. 1: Experimental timeline. | Cell Death & Disease

Fig. 1: Experimental timeline.

From: Intrastriatal injection of Parkinson’s disease intestine and vagus lysates initiates α-synucleinopathy in rat brain

Fig. 1: Experimental timeline.The alternative text for this image may have been generated using AI.

One-month-old male Sprague Dawley rats received unilateral intrastriatal injection of PBS (vehicle control), intestine lysate, or vagus lysate derived from a post-mortem PD patient. The animals injected with PBS (n = 9), intestine lysate (n = 8), or vagus lysate (n = 4) were sacrificed. Paraformaldehyde-fixed tissue sections were processed for IHC and IF to assess both human and rat α-syn accumulation in brain; TH-positive neuron/intensity in SNc, VTA, and STR; and Iba1- and GFAP-positive cells in SNc. HPLC analysis was conducted in the intestine lysate group to assess striatal dopamine levels six months post-injection. DA dopamine, DOPAC 3,4-dihydroxyphenylacetic acid, GFAP glial fibrillary acidic protein, HPLC high-performance liquid chromatography, HVA homovanillic acid, Iba1 ionized calcium-binding adapter molecule 1, IF immunofluorescence, IHC immunohistochemistry, SNc substantia nigra pars compacta, STR striatum, TH tyrosine hydroxylase, VTA ventral tegmental area.

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