Fig. 4: The mesodiencephalic dopaminergic neuronal loss and profound fiber pathology in the rats injected with intestine or vagus lysate. | Cell Death & Disease

Fig. 4: The mesodiencephalic dopaminergic neuronal loss and profound fiber pathology in the rats injected with intestine or vagus lysate.

From: Intrastriatal injection of Parkinson’s disease intestine and vagus lysates initiates α-synucleinopathy in rat brain

Fig. 4: The mesodiencephalic dopaminergic neuronal loss and profound fiber pathology in the rats injected with intestine or vagus lysate.The alternative text for this image may have been generated using AI.

a Representative coronal brain sections of TH immunostaining in the SNc and VTA of rats injected with PBS, intestine lysate, or vagus lysate. The TH-positive dopaminergic neurons of SNc were significantly impaired in the rats with intestine or vagus lysate injection compared to vehicle control. Unpaired t-test, **p = 0.0053 (Intestine); **p = 0.0082 (Vagus). Scale bars, 200 μm. b Representative coronal brain sections of TH immunostaining in the STR of rats injected with PBS, intestine lysate, or vagus lysate. The TH intensity in STR was decreased significantly in the rats with intestine or vagus lysate injection. Unpaired t-test, *p = 0.0376 (Intestine). Scale bars, 100 μm. c HPLC results indicate that the levels of DA and its HVA metabolites significantly decreased in the rats with intestine lysate injection, compared to vehicle control. The changes in DOPAC levels and the ratios of HVA/DA and DOPAC/DA were not obvious. Unpaired t-test, *p = 0.0238 (DA); *p = 0.0439 (HVA). DA dopamine, DOPAC 3,4-dihydroxyphenylacetic acid, HVA homovanillic acid, SNc substantia nigra pars compacta, STR striatum, TH tyrosine hydroxylase, VTA ventral tegmental area.

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