Fig. 3: TPX2 interacts with PXR and affect the nuclear accumulation of PXR in HCC cells. | Cell Death & Disease

Fig. 3: TPX2 interacts with PXR and affect the nuclear accumulation of PXR in HCC cells.

From: TPX2 enhances the transcription factor activation of PXR and enhances the resistance of hepatocellular carcinoma cells to antitumor drugs

Fig. 3: TPX2 interacts with PXR and affect the nuclear accumulation of PXR in HCC cells.

(A and B) The interaction between TPX2 and PXR was identified by “IP: FLAG; IB: PXR” or “IP: FLAG; IB: TPX2”. (C and D) The interaction between FLAG-PXR mutation (including NTD [N-terminal domain, residues 1–40], DBD [DNA-binding domain, residues 41–110], HD [hinge domain, residues 1–140] and LBD [ligand-binding domain, residues 141–434]), and HA-TPX2; FLAG-TPX2 mutations (including 1–45aa, 46–140aa, 141–280aa, 281–320aa, and 321–747aa) and HA-PXR was identified by “IP: FLAG; IB: HA”. (E and F) The HCC cells, MHCC97-L (E), MHCC97-H (F), were co-transfected with plasmids (control or TPX2 for MHCC97-L; control or siTPX2 for MHCC97-H). Cells were treated with solvent control or rifampicin. The protein level of CYP3A4 or P-GP was examined using western blot. (G and H) The HCC cells, MHCC97-L (E), MHCC97-H (F), were co-transfected with plasmids (control or TPX2 for MHCC97-L; control or siTPX2 for MHCC97-H). Cells were treated with solvent control or rifampicin. The cells were separated into nuclear sub-fraction or cytoplasm sub-fraction. The protein level of PXR or TPX2 was examined using western blot.

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