Fig. 4: TPX2 enhances the recruitment of PXR to its downstream gene cyp3a4’s promoter or enhancer regions. | Cell Death & Disease

Fig. 4: TPX2 enhances the recruitment of PXR to its downstream gene cyp3a4’s promoter or enhancer regions.

From: TPX2 enhances the transcription factor activation of PXR and enhances the resistance of hepatocellular carcinoma cells to antitumor drugs

Fig. 4

(A) The downstream gene of PXR, cyp3a4, is upstream of the transcription initiation site, including the promoter region sequence (PXRE) and the enhancer region sequence (XREM). Two PXR binding sites (one DR3 motif and one ER6 motif) are located in XREM in addition to a PXR binding site (DR6 motif) in PXRE. In chromatin co-immunoprecipitation (ChIP), XREM and PXRE sequences were amplified respectively. The HCC cells, MHCC97-L (B and C), MHCC97-H (D and E) and HepG2 (F and G), were transfected with plasmids (control or TPX2 for MHCC97-L; control or siTPX2 for MHCC97-H; control, TPX2 or siTPX2 for HepG2 cells). Cells were treated with solvent control or rifampicin and analyzed using ChIP. P < 0.05.

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