Fig. 8: TPX2 promotes the recruitment of PXR to its downstream gene cyp3a4’s promoter or enhancer region.

Rifampicin was used as an agonist of PXR, and the microtubule aggregation agent, paclitaxel, and the microtubule depolymerizing agent, vincristine, were used in combination with rifampicin. (A) The time points for this step are shown. Using a ChIP assay to detect the recruitment of PXR (B and C) or RXR in the enhancer (XREM) (B and D) and promoter (PXRE) (C and E) regions of cyp3a4. *P < 0.05.