Table 4 The primers used in the qPCR and ChIP assays in the presence work.
Genes | primers | sequences |
|---|---|---|
cyp3a4 | the forward primer | 5’-CTAGCACATCATTTGGACTG-3 |
the reverse primer | 5’-ACAGAGCTTTGTGGGACT-3' | |
PXR | the forward primer | 5’-AGAGCGGCATGAAGAAGGAGATG-3' |
the reverse primer | 5’-GAAATGGGAGAAGGTAGTGTCAAAGG-3' | |
P-gp (mdr-1) | the forward primer | 5’-CCATAGCTCGCGCCCTTGTTAGA-3' |
the reverse primer | 5’-CGGTGAGCAATCACAATGCAG-3' | |
tpx2 | the forward primer | 5′-ACCTTGCCCTACTAAGATT-3′ |
the reverse primer | 5′-AATGTGGCACAGGTTGAGC-3′ | |
β-Actin (loading control) | the forward primer | 5’-CTCCATCCTGGCCTCGCTGT-3' |
the reverse primer | 5’-GCTGTCACCTTCACCGTTCC-3' | |
RXR | the forward primer | 5’-AGATGGACAAGACGGAGCTG-3’ |
the reverse primer | 5’-CCAAGGACGCATAGACCTTC-3’. | |
XREM | the forward primer | 5’-TCTAGAGAGATGGTTCATTCC-3' |
the reverse primer | 5’-TCTTCAACAGGTTAAAGGAG-3' | |
PXRE | the forward primer | 5’-AGATCTGTAGGTGTGGCTTGTTGG-3' |
the reverse primer | 5’-TGTTGCTCTTTGCTGGGCTATGTGC-3' | |
The Input | the forward primer | 5’-AACCTATTAACTCACCCTTGT-3' |
the reverse primer | 5’-CCTCCATTCAAAAGATCTTATTATTTAGCATCTCCT-3' |
