Fig. 5: tPA-containing vesicles show similarities in their anterograde axonal trafficking under basal and activating conditions with VAMP2 positive vesicles. | Cell Death & Disease

Fig. 5: tPA-containing vesicles show similarities in their anterograde axonal trafficking under basal and activating conditions with VAMP2 positive vesicles.

From: Modulations of the neuronal trafficking of tissue-type plasminogen activator (tPA) influences glutamate release

Fig. 5

Representative kymographs of dendritic (upper panel) and axonal (lower panel) tPA-containing vesicles and VAMP2 positives vesicles after treatments: control HBBSS or bicuculline/glycine (Bic/Gly, 30 µM/200 µM respectively). B Kymographs analysis of anterograde and retrograde velocity in µm.s−1 and (C) their respective cumulative distances in dendrites in µm for each condition. Control tPA n = 106; Control VAMP2 n = 106; Bic/Gly tPA n = 154; Bic/Gly VAMP2 n = 155 vesicles for retrograde trafficking and Control tPA n = 177; Control VAMP2 n = 112; Bic/Gly tPA n = 166; Bic/Gly VAMP2 n = 144 vesicles for anterograde trafficking from 10 to 15 neurons from 3 to 5 independent cultures. D Kymographs analysis of anterograde and retrograde velocity in µm.s−1 and (E) their respective cumulative distances in axons in µm for each condition. Control tPA n = 200; Control VAMP2 n = 108; Bic/Gly tPA n = 379; Bic/Gly VAMP2 n = 70 vesicles for retrograde trafficking and Control tPA n = 104; Control VAMP2 n = 168; Bic/Gly tPA n = 185; Bic/Gly VAMP2 n = 136 vesicles for anterograde trafficking from 10 to 15 neurons from 3 to 6 independent cultures. F Representative kymographs of dendritic (high) and axonal (down) tPA-containing vesicles after treatments: control DMSO or cyanquixaline/Acide 2-amino-5-phosphonovalérique (CNQX/APV, 50 mM/50 mM respectively). G Kymographs analysis of anterograde and retrograde velocity in µm.s−1 and (H) their respective cumulative distances in dendrites in µm for each condition. Control tPA n = 181; Control VAMP2 n = 110; CNQX/APV tPA n = 155; CNQX/APV VAMP2 n = 118 vesicles for retrograde trafficking and Control tPA n = 180; Control VAMP2 n = 119; CNQX/APV tPA n = 169; CNQX/APV VAMP2 n = 113 vesicles for anterograde trafficking from 11 to 13 neurons from 2 to 5 independent cultures. I Kymographs analysis of anterograde and retrograde velocity in µm.s−1 and (J) their respective cumulative distances in dendrites in µm for each condition. Control tPA n = 549; Control VAMP2 n = 67; CNQX/APV tPA n = 252; CNQX/APV VAMP2 n = 120 vesicles for retrograde trafficking and Control tPA n = 250; Control VAMP2 n = 140; CNQX/APV tPA n = 81; CNQX/APV VAMP2 n = 219 vesicles for anterograde trafficking from 10 to 17 neurons from 3 to 5 independent cultures. B–E, G–J Kruskal–Wallis’s test followed by a Dunn’s multiple comparisons test were used. Error bars = SD. A, F kymographs scale: x = 40 µm, y = 10 min. *p < 0.05; **p < 0.01; ***p < 0.005; ****p < 0.0001, ns not significant.

Back to article page