Fig. 6: Identification of DDIT4 as a key common target by which the NR modulators inhibited osteosarcoma growth. | Cell Death & Disease

Fig. 6: Identification of DDIT4 as a key common target by which the NR modulators inhibited osteosarcoma growth.

From: Nuclear receptor modulators inhibit osteosarcoma cell proliferation and tumour growth by regulating the mTOR signaling pathway

Fig. 6: Identification of DDIT4 as a key common target by which the NR modulators inhibited osteosarcoma growth.

A Plot shows the four genes that were commonly regulated by the NR modulators (upregulated in black, downregulated in white). B FPKM of the common targeted genes in each group. Mean ± SD, n = 3; *P < 0.05, the NR modulator group versus the Ctrl group. Wt and DDIT4-KO U2OS cells were treated with the RARb antagonist, PPARg antagonist, LXR agonist and Rev-Erba agonist at 10 μM. C The levels of phosphorylated mTOR and S6 were evaluated. D–G Cell growth was evaluated by the IncuCyte zoom living cell imaging system. Mean ± SD, n = 6; *P < 0.05, the DDIT4 KO group versus the Wt group. #P < 0.05, the NR modulator group versus the saline group. △Confluence represents the difference in confluence between the saline group and the NR modulator group. The violin plots show the distribution of the △Confluence of the indicated genotypes. Paired t-tests were used to evaluate the significance. *P < 0.05, the DDIT4 KO group versus the Wt group.

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