Fig. 4: CEMIP promotes angiogenesis by increasing ECM stiffness. | Cell Death & Disease

Fig. 4: CEMIP promotes angiogenesis by increasing ECM stiffness.

From: Inhibition of CEMIP potentiates the effect of sorafenib on metastatic hepatocellular carcinoma by reducing the stiffness of lung metastases

Fig. 4

A Stiffness maps and stiffness distribution of lung tissues were measured using AFM. Lung stiffness was significantly elevated in the 3-week-pre-metastatic niche and was highest in 5-week-lung metastases. B Comparison of wave speeds among 0-week, 3-week, and 5-week groups via SWE measurement ***P < 0.001. C Left: Normal CT appearance. Middle: The predominant abnormality is patchy and exhibits bilateral ground-glass opacification, peribronchial consolidation (blue triangle), and tractional bronchiectasis (red arrows), and its appearance was consistent with that of fibrosis. Right: Sagittal reformation shows numerous thickened interlobular septa (green circle), peripheral or peribronchial consolidation, and mild reticular changes representative of typical lung fibrosis. D Immunofluorescent staining of COL-I (red), α-SMA (green), and CEMIP (pink) on paraffin-processed lung tissue sections. Scale bar = 50 μm. E CD34 staining of paraffin sections of lung tissues obtained from nude mice. Scale bar = 50 μm. F Representative images of tube formation in HUVECs cultured on Matrigel-coated plates with CEMIP-overexpressing lentivirus treated with pirfenidone/sorafenib.

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