Fig. 1: Single myofiber gene signatures reveal the transcriptional changes induced by SMN loss in skeletal muscle. | Cell Death & Disease

Fig. 1: Single myofiber gene signatures reveal the transcriptional changes induced by SMN loss in skeletal muscle.

From: Dysfunctional mitochondria accumulate in a skeletal muscle knockout model of Smn1, the causal gene of spinal muscular atrophy

Fig. 1

a Schematic representation of the experimental steps leading to microarrays of cDNA produced from RNA extracted from single myofibers of the indicated genotypes. b Cluster analysis of the differentially expressed genes (DEGs) distinguishes the signatures of myofibers isolated from tibialis anterior muscles of Wt and Smn1ΔSkm mice. Gene ontology (GO) analysis of over-and under-expressed genes in response to SMN loss. Enrichment score by DAVID was used to rank the annotation groups. The numbers inside bars correspond to the number of DEGs in each cluster. c Plot of the Fisher score (y-axis) vs. the Z-score (x-axis) from the oPOSSUM transcription factor enrichment analysis of the list of DEGs in b. Each transcription factor is represented by a dot on the graph; transcription factors whose binding sites are most strongly enriched within the promoters of the specified gene list have high scores (top right of plot).

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