Fig. 5: Activated PKG I bound with EGFR leading its serine and threonine phosphorylation. | Cell Death & Disease

Fig. 5: Activated PKG I bound with EGFR leading its serine and threonine phosphorylation.

From: Increased endogenous PKG I activity attenuates EGF-induced proliferation and migration of epithelial ovarian cancer via the MAPK/ERK pathway

Fig. 5

A Confocal laser scanning microscopy images on the expressions of EGFR and PKG I in SKOV3 and A2780 cells. Scale bar, 5 μm. B Co-IP assay revealed that PKG I bounded with EGFR in SKOV3 and A2780 cells. C Immunoprecipitation assay the serine and threonine phosphorylation of EGFR in SKOV3 and A2780 cells. D Mass spectrometry analysis identified that activated PKG I caused threonine 693 phosphorylation of EGFR. E Western blot analysis showed that activated PKG I caused phosphorylation of EGFR threonine 693 site in SKOV3 and A2780 cells.

Back to article page