Table 1 Primers used for the amplification of human transcripts by real-time quantitative PCR.

From: Interleukin-18-primed human umbilical cord-mesenchymal stem cells achieve superior therapeutic efficacy for severe viral pneumonia via enhancing T-cell immunosuppression

Genes

Forward sequence (5’ to 3’)

Reverse sequence (5’ to 3’)

GAPDH

GTCTCCTCTGACTTCAACAGCG

ACCACCCTGTTGCTGTAGCCAA

VCAM1

GATTCTGTGCCCACAGTAAGGC

TGGTCACAGAGCCACCTTCTTG

ICAM1

AGCGGCTGACGTGTGCAGTAAT

TCTGAGACCTCTGGCTTCGTCA

MMP1

ATGAAGCAGCCCAGATGTGGAG

TGGTCCACATCTGCTCTTGGCA

MMP2

AGCGAGTGGATGCCGCCTTTAA

CATTCCAGGCATCTGCGATGAG

CCL2 (MCP1)

AGAATCACCAGCAGCAAGTGTCC

TCCTGAACCCACTTCTGCTTGG

CCL5 (RANTES)

CCTGCTGCTTTGCCTACATTGC

ACACACTTGGCGGTTCTTTCGG

CCL7 (MCP3)

ACAGAAGGACCACCAGTAGCCA

GGTGCTTCATAAAGTCCTGGACC

CXCL1 (GRO α)

AGCTTGCCTCAATCCTGCATCC

TCCTTCAGGAACAGCCACCAGT

CXCL2 (GRO β)

GGCAGAAAGCTTGTCTCAACCC

CTCCTTCAGGAACAGCCACCAA

CXCL5

CAGACCACGCAAGGAGTTCATC

TTCCTTCCCGTTCTTCAGGGAG

CXCL8 (IL8)

GAGAGTGATTGAGAGTGGACCAC

CACAACCCTCTGCACCCAGTTT

CXCL12 (SDF1)

CTCAACACTCCAAACTGTGCCC

CTCCAGGTACTCCTGAATCCAC

NGF

ACCCGCAACATTACTGTGGACC

GACCTCGAAGTCCAGATCCTGA

TGF-β1

TACCTGAACCCGTGTTGCTCTC

GTTGCTGAGGTATCGCCAGGAA

IGF1

CTCTTCAGTTCGTGTGTGGAGAC

CAGCCTCCTTAGATCACAGCTC

EGF

TGCGATGCCAAGCAGTCTGTGA

GCATAGCCCAATCTGAGAACCAC

FGF2

AGCGGCTGTACTGCAAAAACGG

CCTTTGATAGACACAACTCCTCTC

HGF

GAGAGTTGGGTTCTTACTGCACG

CTCATCTCCTCTTCCGTGGACA

IDO1

GCCTGATCTCATAGAGTCTGGC

TGCATCCCAGAACTAGACGTGC

PGE2

TCAAGATGTACGTGGTGGCC

CAGAAAGGAGTAGACGAAGCC

TSG6 (TNFAIP6)

TCACCTACGCAGAAGCTAAGGC

TCCAACTCTGCCCTTAGCCATC

PD-L1 (CD274)

TGCCGACTACAAGCGAATTACTG

CTGCTTGTCCAGATGACTTCGG

HLA-G

GAAGAGGAGACACGGAACACCA

TCGCAGCCAATCATCCACTGGA

PTGES-2

CCTCTATGAGGCTGCTGACAAG

ATCACACGCAGCACGCCATACA

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