Fig. 1: USP49 depletion impairs cell proliferation.
From: USP49 deubiquitinase regulates the mitotic spindle checkpoint and prevents aneuploidy
A MTT proliferation assay in HEK-293T (upper left), HeLa (upper middle), HCT116 (upper right), U2OS (lower left), NCI-H661 (lower middle), and MDA-MB-231 (lower right) cell lines transduced with control (pLKO.1) or three USP49-specific shRNAs (shUSP49.76, shUSP49.77, and shUSP49.79). Data are presented as mean ± SEM and statistical significance was assessed by using one-way ANOVA test (pLKO.1 set as the control column). All data were obtained from 3 independent experiments. B USP49 shRNA effects on HCT116 proliferation were rescued by the overexpression of both USP49Long and USP49Short resistant cDNAs. Normalized cell growth at day 4 upon overexpression is presented in the bar graph. Data are presented as mean ± SEM and statistical significance was assessed by using one-way ANOVA test (pCDH/shUSP49 set as the control column). All data were obtained from 3 independent experiments.
