Fig. 6: LINC00941 recruits ILF2 and YBX1 onto SOX2 promoter to activate its expression. | Cell Death & Disease

Fig. 6: LINC00941 recruits ILF2 and YBX1 onto SOX2 promoter to activate its expression.

From: Reciprocal regulation of LINC00941 and SOX2 promotes progression of esophageal squamous cell carcinoma

Fig. 6: LINC00941 recruits ILF2 and YBX1 onto SOX2 promoter to activate its expression.The alternative text for this image may have been generated using AI.

A Schematics show the putative YBX1-binding site on the SOX2 promoter region. B, C The mRNA and protein expression levels of SOX2 were detected by qRT-PCR (B) and immunoblot (C). D The association between ILF2 and YBX1 was detected in the indicated cells by co-immunoprecipitation assay using anti-ILF2 followed by immunoblot with anti-ILF2 or anti-YBX1. E The subcellular localization of YBX1 protein was confirmed by immunofluorescence assay in the indicated cells. White arrows indicated representative YBX1 expression in the nucleus. Nuclei are stained with DAPI. Scale bars, 50 μm. F The binding of YBX1 on the SOX2 promoter in the indicated cells was assessed by ChIP-qPCR assay using anti-YBX1 antibodies or IgG control. G, H The activity of the SOX2 promoter in the indicated cells was assessed by a dual-luciferase reporter assay. *P < 0.05 compared with the control cells; #P < 0.05 compared with YBX1 + siControl. The experiments are representative of three independent experiments with similar results.

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