Fig. 5: WM-3835 inhibits H3-H4 acetylation and expression of several pro-cancerous genes in primary CRPC cells. | Cell Death & Disease

Fig. 5: WM-3835 inhibits H3-H4 acetylation and expression of several pro-cancerous genes in primary CRPC cells.

From: A first-in-class HBO1 inhibitor WM-3835 inhibits castration-resistant prostate cancer cell growth in vitro and in vivo

Fig. 5: WM-3835 inhibits H3-H4 acetylation and expression of several pro-cancerous genes in primary CRPC cells.The alternative text for this image may have been generated using AI.

The primary pPC-1 cells were treated with WM-3835 (10 μM) or vehicle control (“Veh”) for designated hours, expression of listed proteins and mRNAs were tested (A and B). The stable primary pPC-1 cells with HBO1 shRNA (“shHBO1”) or the scramble control shRNA (“shC”) were further treated with or without WM-3835 (10 μM) for designated hours, expression of listed proteins and mRNAs were tested (C and D); The primary pPC-1 cells, with the lentiviral HBO1-expressing construct (oeHBO1-Slc-1 and oeHBO1-Slc-2, two stable selections) or the empty vector (“Vec”) were established, expression of listed proteins and mRNAs were tested (E and F); Data were expressed as the mean ± standard deviation (SD, n = 5). *P < 0.05 versus “Veh”/“shC”/“Vec” group. “n. s.” stands for non-statistical difference (P > 0.05).

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