Fig. 3: TINCR recruits USP20 to stabilize PD-L1. | Cell Death & Disease

Fig. 3: TINCR recruits USP20 to stabilize PD-L1.

From: LncRNA TINCR impairs the efficacy of immunotherapy against breast cancer by recruiting DNMT1 and downregulating MiR-199a-5p via the STAT1–TINCR-USP20-PD-L1 axis

Fig. 3

A Transcriptome sequencing in T47D cells after TINCR knockdown. B, C RNA expression of different deubiquitinases after TINCR knockdown in MDA-MB-231 and UACC812 cells. D USP20 protein expression after TINCR knockdown. E, F PD-L1 protein expression after USP20 knockdown. PD-L1 expression in (G) mRNA and (H) protein levels after USP20 overexpression in MDA-MB-231 cells. I, J Detection of PD-L1 expression after TINCR knockdown and USP20 overexpression. K CO-IP experiments confirmed that USP20 binds to PD-L1. L Ubiquitination assay of PD-L1 in control, USP20-knocked, and USP20-overexpressing HEK293T cells co-transfected with Ub and treated with MG132. Data are presented as means from three independent experiments ± S.D. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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