Fig. 4: MiR-199a-5p acts as a molecular sponge to mediate the upregulation of USP20 expression by TINCR. | Cell Death & Disease

Fig. 4: MiR-199a-5p acts as a molecular sponge to mediate the upregulation of USP20 expression by TINCR.

From: LncRNA TINCR impairs the efficacy of immunotherapy against breast cancer by recruiting DNMT1 and downregulating MiR-199a-5p via the STAT1–TINCR-USP20-PD-L1 axis

Fig. 4

A Subcellular localization of TINCR in breast cancer cell lines assessed by nuclear/cytoplasmic extract isolation assay. B Overlapping miRNAs in transcriptome miRNA sequencing data from the HMUCC cohort and TargetScan. C Expression of miRNAs with TINCR knockdown in UACC812 cells. D–G Expression of TINCR, USP20, and PD-L1. Complementarity between the miR-199a-5p seed sequence and 3ʹ-UTRs of (H) TINCR and (I) USP20 by using TargetScan and StarBase online databases. Luciferase-reporter assay assessing the interactions between miR-199a-5p and its binding sites or mutated binding sites in 3ʹ-UTRs of (J) TINCR and (K) USP20 in HEK293T cells. L–O RNA immunoprecipitation with a monoclonal anti-Ago2 antibody was used to assess endogenous Ago2 binding to RNA; IgG was used as the control. The levels of TINCR, DNMT1, and miR-199a-5p were determined by qRT-PCR and presented as fold enrichment in Ago2 relative to input. Data are presented as means from three independent experiments ± S.D. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Back to article page