Fig. 5: TINCR recruits DNMT1 to miR-199a-5p locus and suppresses its expression via DNA methylation. | Cell Death & Disease

Fig. 5: TINCR recruits DNMT1 to miR-199a-5p locus and suppresses its expression via DNA methylation.

From: LncRNA TINCR impairs the efficacy of immunotherapy against breast cancer by recruiting DNMT1 and downregulating MiR-199a-5p via the STAT1–TINCR-USP20-PD-L1 axis

Fig. 5

A Expression of pri-miR-199a-5p and pre-miR-199a-5p was detected by qRT-PCR after TINCR knockdown. B The predicted CpG island in the miR-199a-5p locus. Expression levels of (C) pri-miR-199a-5p, pre-miR-199a-5p and (D) miR-199a-5p were detected by qRT-PCR after stimulation with the DNA methyltransferase inhibitor 5AZA. Expression of (E) pri-miR-199a-5p, pre-miR-199a-5p and (F) miR-199a-5p was detected by qRT-PCR after DNMT1 knockdown. G RIP-assay to detect the enrichment of DNMT1 on TINCR. IgG: negative control. H–J ChIP assay to detect the enrichment of DNMT1 at the promoter of the miR-199a-5p locus. K The expression of miR-199a-5p, pri-miR-199a-5p and pre-miR-199a-5p was detected by qPCR after DNMT1 knockdown and TINCR overexpression. Data are presented as means from three independent experiments ± S.D. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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