Fig. 6: IFN-γ promotes TINCR expression by upregulating STAT1. | Cell Death & Disease

Fig. 6: IFN-γ promotes TINCR expression by upregulating STAT1.

From: LncRNA TINCR impairs the efficacy of immunotherapy against breast cancer by recruiting DNMT1 and downregulating MiR-199a-5p via the STAT1–TINCR-USP20-PD-L1 axis

Fig. 6

A Tumorigenesis and tumor tissues in each group of Balb/c mice (n = 6/group). B–D Tumor growth, weight, and number of nodules in the lungs of Balb/c mice per group. E–I Expression of STAT1, TINCR, USP20, and PD-L1, after IFN-γ stimulation. J–L TINCR and STAT1 were detected using qRT-PCR and western after STAT1 knockdown. M, N ChIP and luciferase-reporter assay were used to verify the interaction between TINCR and STAT1. O, P Expression of STAT1 in breast cancer tissues by immunohistochemistry; scale bar, 400 μm. Q Expression of STAT1, p-STAT1, USP20, and PD-L1 in mouse tissues of the scramble and TINCR knockdown groups (n = 6/group). Data are presented as means from three independent experiments ± S.D. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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