Fig. 4: ET-1 promotes the phosphorylation of talin1 and its association with active Intβ1. | Cell Death & Disease

Fig. 4: ET-1 promotes the phosphorylation of talin1 and its association with active Intβ1.

From: The interaction of β-arrestin1 with talin1 driven by endothelin A receptor as a feature of α5β1 integrin activation in high-grade serous ovarian cancer

Fig. 4: ET-1 promotes the phosphorylation of talin1 and its association with active Intβ1.The alternative text for this image may have been generated using AI.

A CLSM analysis of SKOV3 cells stimulated with ET-1 for 5 min and/or AMB and/or ATN161, then stained for active Intβ1 (green) and talin1 (red) detection. Colocalization is shown in merged images, detected in yellow. For ET-1 stimulation, intracellular accumulation of active Intβ1 is depicted by arrows and higher-power magnification images of two selected ROI are shown, bringing out active Intβ1/talin1 colocalization. Nuclei are reported in blue (DAPI). Scale bar, 20 μm. Columns show the mean ± SD of quantification of Pearson’s correlation between active Intβ1 and talin1. B CLSM analysis of SKOV3 cells stimulated with ET-1 and/or AMB and/or ATN161 for 5 min and stained for active Intβ1 (green) and ptalin1 (red). Colocalization is shown in merged images, detected in yellow and depicted by an arrow. Nuclei are reported in blue (DAPI). Scale bar, 20 μm. Columns, mean ± SD of quantification of Pearson’s correlation between active Intβ1 and ptalin1. n = 3, one-way ANOVA.

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