Fig. 7: HG-SOC invasion is regulated by ET-1/Intβ1 signaling. | Cell Death & Disease

Fig. 7: HG-SOC invasion is regulated by ET-1/Intβ1 signaling.

From: The interaction of β-arrestin1 with talin1 driven by endothelin A receptor as a feature of α5β1 integrin activation in high-grade serous ovarian cancer

Fig. 7: HG-SOC invasion is regulated by ET-1/Intβ1 signaling.The alt text for this image may have been generated using AI.

A si-SCR, or si-TLN1 or si-ARRB1 OVCAR3 cells treated with ET-1 and/or AMB and/or ATN161 were allowed to invade fibronectin/type I collagen plugs in an inverted invasion assay (48 h). Cells were stained with PKH67, and serial optical sections (10 μm intervals) were acquired. The invasion was measured by dividing the sum of signal intensity of all slides beyond 20 μm (invading cells) by the sum of the intensity of all slides (total cells). n = 2, one-way ANOVA, Tukey post hoc analysis. Scale bar, 200 µm. B SKOV3 cells plated on a monolayer of MCs grown on fibronectin/type I collagen in a polystyrene scaffold were allowed to invade for 7 days in the absence and presence of ET-1 and/or AMB and/or ATN161, then fixed with Bouin’s solution and paraffin-embedded scaffolds were then cut into thin slices (10 µm). The images show cell invasion in a 3D organotypic model. Hematoxylin and eosin staining are shown. C Sections as in (B) were stained for active Intβ1 (green) and DAPI (blue) detection. The corresponding transmitted light images are also shown. Arrows depict the top side of the scaffold where cells were plated. Scale bar, 50 µm.

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