Fig. 8: Ambrisentan as well as ATN161 controls SOC cell metastatic colonization . | Cell Death & Disease

Fig. 8: Ambrisentan as well as ATN161 controls SOC cell metastatic colonization .

From: The interaction of β-arrestin1 with talin1 driven by endothelin A receptor as a feature of α5β1 integrin activation in high-grade serous ovarian cancer

Fig. 8: Ambrisentan as well as ATN161 controls SOC cell metastatic colonization .The alt text for this image may have been generated using AI.

A In vivo adhesion assays as showed by bioluminescent images of SKOV3-Luc cells, untreated (CTR) or pretreated with AMB or ATN161 or with a combination, on abdominal organs (n = 5 mice/group). The organs arranged are the intestine and mesentery, the spleen with the pancreas, and omentum. The adherent cell aggregates were observed, and the luminescence was read and recorded. n = 2 one-way ANOVA. B Bioluminescent images of intraperitoneally (i.p.) injected SKOV3-Luc cells (2 × 106 cells) in NOD/SCID mice, undergoing treatments for 5 weeks with 200 L Metocell (vehicle, CTR) or 200 μL AMB (10 mg/kg, oral daily), both by oral gavage, or ATN161 (100 μg/kg, i.p. twice a week). Tumor burden was assessed on days 17, 25, 32, and 39 after tumor cell injection. Data are presented as mean ± SD, n = 2, one-way ANOVA. C Representative WB for Intβ1 expression in metastatic nodules. GAPDH was used for loading control. D Representative CLSM images of paraffin-embedded formalin-fixed tumor tissue sections stained for active Intβ1 from mice as in (B), stained for active Intβ1 (green). Nuclei in the tissue sections were counterstained with DAPI. Scale bar = 50 µm.

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