Fig. 6: GDCA upregulated ZBP1 expression in mouse BMDMs in a time- and dose-dependent manner. | Cell Death & Disease

Fig. 6: GDCA upregulated ZBP1 expression in mouse BMDMs in a time- and dose-dependent manner.

From: Necroptosis of macrophage is a key pathological feature in biliary atresia via GDCA/S1PR2/ZBP1/p-MLKL axis

Fig. 6

A, B ZBP1 mRNA (A) and protein (B) levels were measured by qRT-PCR and western blot in BMDMs treated with 100 μmol/L sodium taurocholate hydrate (TCA), glycocholic acid hydrate (GCA), and sodium glycodeoxycholate (GDCA) for 6 h, respectively. C Zbp1 mRNA level was measured by qRT-PCR in mouse BMDMs treated with the indicated concentrations of GDCA for 6 h. D Zbp1 mRNA level was measured by qRT-PCR in mouse BMDMs treated with 100 μmol/L GDCA for different time points. E, F ZBP1 protein level was measured in mouse BMDMs treated with the indicated concentrations of GDCA (E) or different time points (F). All results were confirmed by at least three independent experiments. Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (versus control).

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