Fig. 5: RBM3 inhibits osteoblast-induced Wnt/β-catenin activation in PCa cells by inducing CTNNB1 m6A methylation. | Cell Death & Disease

Fig. 5: RBM3 inhibits osteoblast-induced Wnt/β-catenin activation in PCa cells by inducing CTNNB1 m6A methylation.

From: RBM3 suppresses stemness remodeling of prostate cancer in bone microenvironment by modulating N6-methyladenosine on CTNNB1 mRNA

Fig. 5

a FISH staining of the expression and subcellular localization of CTNNB1 mRNA in three prostate cancer cells with or without osteoblast co-culture. Cells were counterstained with 4’, 6-diamidino-2-phenylindole (DAPI) to show nuclei; scale bar, 20 µm. b SELECT m6A site identification detection system was used to detect the m6A modification level of CTNNB1 Site1 in prostate cancer cells with or without osteoblast co-culture. The curve in the figure is the amplification curve, and the histogram is the fold change of m6A modification level calculated by 2-ΔΔCT. Data are mean ± SD (n = 3); *P < 0.05, **P < 0.01. P values were calculated using two-sided paired Student’s t test. c FISH and IF co-staining of the expression and subcellular localization of CTNNB1 mRNA and RBM3 protein in RBM3 overexpressing PCa cells with or without osteoblast co-culture. Cells were counterstained with 4’, 6-diamidino-2-phenylindole (DAPI) to show nuclei; scale bar, 20 µm.

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