Fig. 4: Restoration of BIRC2 expression reverses the inhibitory effect of BRD7 on cell proliferation. | Cell Death & Disease

Fig. 4: Restoration of BIRC2 expression reverses the inhibitory effect of BRD7 on cell proliferation.

From: BRD7 inhibits enhancer activity and expression of BIRC2 to suppress tumor growth and metastasis in nasopharyngeal carcinoma

Fig. 4

A Western blotting was performed to confirm BRD7 and BIRC2 protein levels using antibodies against BRD7 and BIRC2. GAPDH served as an internal control. (B) and (C) CCK-8 analysis and colony-forming assays of cell proliferation in 5-8 F and HNE1 cells stably with BRD7 overexpression, BRD7 and BIRC2 simultaneous overexpression or control group. D Cell-cycle analysis and cell apoptosis by flow cytometry. E Significantly differently expressed proteins involved in cell cycle progression (P21, CCND1 and CDK4) and survival (Total PARP, Cleaved PARP and Bcl2) in BRD7 overexpression, BIRC2 overexpression and BIRC2 restoration cells, respectively. GAPDH served as an internal control. Error bars represent the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001; NS, no significance. All experiments were performed in triplicate.

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