Fig. 5: UBE2E2 regulates Nrf2 expression and nuclear translocation. | Cell Death & Disease

Fig. 5: UBE2E2 regulates Nrf2 expression and nuclear translocation.

From: UBE2E2 enhances Snail-mediated epithelial-mesenchymal transition and Nrf2-mediated antioxidant activity in ovarian cancer

Fig. 5

A, B The expression of Nrf2 pathway proteins was explored by western blotting with β-actin as the loading control. MG132, which is used to block the proteasome system, was added to the cultures (final concentration of 10 μM) 12 h prior to cell harvesting. C The subcellular distribution of Nrf2 was assessed by immunofluorescence staining. An overlay of blue (nuclei) and red (Nrf2) channels is shown (scale bar = 20 μm). D The cytoplasm and nuclear fractions were analyzed by immunoblotting with β-tubulin, and Lamin B1 as markers for cytosol and nuclear fractions, respectively. E The expression of Nrf2 pathway proteins was detected by immunoblotting. F The mRNA levels were measured by qRT–PCR (*p < 0.05; n.s. no significant differences detected). G The mRNA expression level of Nrf2 in OvCa cells was measured by qRT–PCR. H Immunoprecipitation (IP) of Flag-tagged UBE2E2 in SKOV3 cells.

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