Fig. 6: The N-terminal of UBE2E2 is required for its interaction with Nrf2. | Cell Death & Disease

Fig. 6: The N-terminal of UBE2E2 is required for its interaction with Nrf2.

From: UBE2E2 enhances Snail-mediated epithelial-mesenchymal transition and Nrf2-mediated antioxidant activity in ovarian cancer

Fig. 6

A Schematic diagram showing the full-length and truncated forms of UBE2E2. The numbers indicate the positions of the amino acids. WT wild-type. B Effects of the truncation mutation on the UBE2E2 intracellular distribution. An overlay of blue (nuclei) and green (GFP) channels is shown. Scale bar = 20 μm. C Effects of mutations on the UBE2E2 intracellular distribution in SKOV3 cells. An overlay of blue (nuclei) and green (GFP) channels is shown (scale bar = 20 μm). D The expression of Nrf2, Flag and UBE2E2 was explored by western blot analysis 72 h after transfection with WT or mutant UBE2E2 expression vectors. MG132 was added to the cultures 12 h prior to cell harvesting. β-actin was used as the loading control. Δ53 Δ53-201. E Immunoprecipitation (IP) of Flag-tagged WT or mutant UBE2E2 in OvCa cells.

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