Fig. 5: Leptin-upregulated FAO was responsible for inflammatory phenotypes of RA-FLS. | Cell Death & Disease

Fig. 5: Leptin-upregulated FAO was responsible for inflammatory phenotypes of RA-FLS.

From: Elevated fatty acid β-oxidation by leptin contributes to the proinflammatory characteristics of fibroblast-like synoviocytes from RA patients via LKB1-AMPK pathway

Fig. 5

RA-FLS were handled by vehicle, leptin (100 ng/mL) with or without ETO (100 μM) for 24 h. RA-FLS were labeled with CFSE and analyzed by flow cytometry for proliferation (A, n = 4). B Expression of IL-6, IL-1β and TNF-α in RA-FLS was tested with real-time PCR analysis (n = 5). C Transwell migration assay was carried out to evaluate migration potential of RA-FLS (scale bar = 50 μm, n = 4). D Supernatant derived from RA-FLS with indicated treatment was added into the culture system of HUVEC. Representative images of capillary-like structures and quantitative analysis of the total tube length were examined at 6 h (scale bar = 100 μm, n = 4). Expression of VEGF was evaluated by real-time PCR at 24 h. Data are means ± SEM of three independent experiments, statistical significance was determined as ***P < 0.001, **P < 0.01, *P < 0.05 compared with control.

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