Fig. 6: CEMIP promotes metastasis of CRC cells through GRAF1/CDC42/MAPK pathway-regulated EMT.

A Western blotting analysis of EMT associated proteins in SW480 cells transfected with the indicated constructs. B Western blotting analysis of EMT associated proteins in SW480 cells transfected with the indicated constructs. C Transwell chamber migration and invasion of SW480 cells transfected with the indicated constructs with or without ZCL278. ***P < 0.001; ****P < 0.0001. D Representative images of livers of BALB/c nude mice, treated with or without ZCL278. Numbers of liver metastases were detected in each group (day 28, n = 5), mice were treated with or without ZCL278. *P < 0.05; **P < 0.01. E Body weight curves of mice treated with or without ZCL278. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. F Survival curves of mice treated with or without ZCL278. *P < 0.05; **P < 0.01.