Fig. 2: CDK5RAP2 expression is regulated by the Wnt signaling pathway.
From: CDK5RAP2 is a Wnt target gene and promotes stemness and progression of oral squamous cell carcinoma
A, B HOK cells were treated with 25 μM ICG-001 A or 100 ng/mL Wnt3a B for 24 h and then cell lysates were immunoblotted with the indicated antibodies. CDK5RAP2 protein levels were quantified and normalized to the β-actin protein levels. C ChIP assay was performed using rabbit anti-CBP antibody or normal rabbit IgG. RPE1 cells were treated with DMSO as mock treatment or 25 μM ICG-001. D Schematic of predicted CBP-binding sites in the cdk5rap2 promoter region. Binding between CBP and the cdk5rap2 promoter was determined using a dual-luciferase reporter assay. RPE1 cells transfected with distinct promotor fragments were treated with or without ICG-001. Firefly luciferase intensity was normalized relative to Renilla luciferase intensity. Data are presented as means ± SD; ns no significance, **P < 0.01, ***P < 0.001, two-tailed Student’s t-test.
