Fig. 6: CDK5RAP2 regulates spindle orientation.
From: CDK5RAP2 is a Wnt target gene and promotes stemness and progression of oral squamous cell carcinoma
A Schematic depicting calculation of spindle angles. The horizontal distance (X μm) and the vertical distance (Z μm) between two spindle poles of metaphase cells were measured by acquiring Z-stack images. Spindle angle (αο), the angle between the spindle axis and the substrate plane, was calculated using an inverse trigonometric function. B Representative Z-stack images of metaphase cells. HeLa cells were fixed and immunostained with anti-α-tubulin and anti-γ-tubulin antibodies. White arrows indicate the focal planes of two spindle poles in two different sections. Scale bars, 5 μm. The spindle angle of HeLa cells after control or CDK5RAP2 knockdown was quantified. Data are presented as means ± SD; *P < 0.05, two-tailed Student’s t-test.
