Fig. 3: Dnali1−/− males display asthenozoospermia (ASZ). | Cell Death & Disease

Fig. 3: Dnali1−/− males display asthenozoospermia (ASZ).

From: DNALI1 deficiency causes male infertility with severe asthenozoospermia in humans and mice by disrupting the assembly of the flagellar inner dynein arms and fibrous sheath

Fig. 3: Dnali1−/− males display asthenozoospermia (ASZ).The alternative text for this image may have been generated using AI.

A A summary of the CRISPR/Cas9 knockout targeting approach. B The PCR identification of mouse genotypes. Primer sites are shown in (A). F\R1 is used for wildtype sequence and F\R2 for knockout sequence. C Western blotting verifying that testis samples from Dnali1−/− mice do not produce a band of the expected size (28 kDa). β-TUBULIN (β-TUB) was used as a normalization control. D IF analysis of spermatozoa from the Dnali1+/+ and Dnali1−/− mice using an anti-DNALI1 antibody. Asterisks indicate the complete loss of DNALI1. E Testes from 9-week-old Dnali1+/+ and Dnali1−/− mice. F Mean testis weight adjusted with body weight, Data are presented as means ± SEM (n = 8 vs. 5; NS, P > 0.05, Student’s t-test). G H&E-stained spermatozoa from the Dnali1+/+ and Dnali1−/− mice. H Mean frequencies of the motile sperm in the Dnali1+/+ and Dnali1−/− mice, (n = 5 vs. 5; ****P < 0.0001, Student’s t-test). I Frequencies of sperm with aberrant morphological profiles in the Dnali1+/+ and Dnali1−/− mice (n = 5 vs. 5; NS, P > 0.05, Student’s t-test). J and K H&E-stained testis (J) and epididymis (K) sections from the Dnali1+/+ and Dnali1−/− mice.

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