Fig. 4: The LCs position of the Dnali1−/− spermatozoa is asymmetrical and the IDA components are incomplete. | Cell Death & Disease

Fig. 4: The LCs position of the Dnali1−/− spermatozoa is asymmetrical and the IDA components are incomplete.

From: DNALI1 deficiency causes male infertility with severe asthenozoospermia in humans and mice by disrupting the assembly of the flagellar inner dynein arms and fibrous sheath

Fig. 4: The LCs position of the Dnali1−/− spermatozoa is asymmetrical and the IDA components are incomplete.The alternative text for this image may have been generated using AI.

A and B The flagellar cross-sectional structure of Dnali1+/+ and Dnali1−/−, as evidenced by TEM. The red arrow indicates an obvious IDA. The numbers represent the quantity of peripheral microtubules. A yellow dash marks the LCs, which are diagonally positioned in the Dnali1+/+ spermatozoa. C The co-staining and the co-localization analysis of AKAP3 and βTUBULIN (β-TUB). The red lines indicate the area where the fluorescence intensity are measured. D Quantitative co-localization analysis of AKAP3 and β-TUB. E IF staining against AKAP4. F–I IF staining depicting the locations of each DNAH proteins in the Dnali1+/+ and Dnali1−/− mice. The asterisks indicate the miss of DNAH1 and DNAH7, respectively.

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