Fig. 5: Identification of differential expressed genes in STAT3 knock down in PCMs.

A Workflow of RNA sequencing in PCMs. PCMs were transiently transfected by siRNA targeting STAT3 (siSTAT3#1) to deplete the endogenous STAT3 level or a scrambled siRNA used as controls at 48 h. RNAs were extracted for next gen-sequencing. B PCMs were transiently transfected with siSTAT3#1 or scrambled siRNA as control. Lysates were collecting at 48 h. Equal amounts of proteins were assessed by western blotting analysis to confirm siRNA mediated STAT3 depletion as compared to scrambled controls. Data are representative of n = 3 biological replicates. C Genes identified by RNA-seq analysis that are upregulated (Red) or downregulated (Blue) in STAT3 depleted PCMs (FDR < 0.05). D A volcano plot indicates upregulated and downregulated genes in siSTAT3 RNA-seq data. Black data point if FDR value >0.01. Red data points if FDR value < 0.01. Labels of the genes are shown if −1.35 > logFC > 2 and FDR value <0.01.