Fig. 4: Notch3 is regulating a Mybl2 signature in mammary gland tumors.
From: Notch3 regulates Mybl2 via HeyL to limit proliferation and tumor initiation in breast cancer
A GSEA analyses of E2F target, G2/M Checkpoint and Mybl2 signature comparing Notch3 wt and Notch3 null (Notch3lacZ/lacZ) tumors in MMTV-Neu induced tumors. B Immunohistochemistry for Mybl2 protein in Notch3 wild type and null tumors. C mRNA expression of E2F1 and Mybl2 targets in tumors dissected from MMTV-NEU; Notch3+/+ (+/+), MMTV-NEU; Notch3lacZ/+ (+/–) and MMTV-NEU; Notch3lacZ/lacZ tumors (–/–). D mRNA q-RT PCR analysis of Mybl2 and target genes in organoïds expressing N3ICD upon doxycyclin treatment (D1). Expression is expressed as fold change between N3ICD expressing and control organoïds of mRNA expression standardize to HPRT1.
