Fig. 2: Effects of stable SHMT2 knockdown on proliferation and metastasis in ccRCC cells. | Cell Death & Disease

Fig. 2: Effects of stable SHMT2 knockdown on proliferation and metastasis in ccRCC cells.

From: Serine hydroxymethyltransferase 2 knockdown induces apoptosis in ccRCC by causing lysosomal membrane permeabilization via metabolic reprogramming

Fig. 2: Effects of stable SHMT2 knockdown on proliferation and metastasis in ccRCC cells.

Lentivirus encoding scramble control shNC or targeted shSHMT2 vectors were infected into ACHN cells. A Western blot analysis was performed to evaluate SHMT2 protein levels in negative control (shNC) or shSHMT2 #1/#2 cells. B Cell proliferation assay of ACHN cells stably transfected with shNC or shSHMT2 #1/#2. Error bars represent SD (n = 3). C Colony formation assays of ACHN cells stably transfected with shNC or shSHMT2 #1/#2 for 10 days. Each experiment was performed in triplicate. D, E Cell migration (D) and invasion (E) assays of ACHN cells stably transfected with shNC or shSHMT2 #1/#2. F Would healing assay of ACHN cells stably transfected with shNC or shSHMT2 #1/#2. G The tumor volume, weight, and images of shNC and shSHMT2 #1/#2 ACHN xenograft in nude mice. Tumor sizes were measured every 3 days for 4 weeks (top panel). Tumor weights were measured after dissection (middle panel). Tumor images were taken after dissection (bottom panel). Data were represented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

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