Fig. 3: The Hoxd13 mutation promoted the osteoclast differentiation.

A The effect of Hoxd13 mutation on the expression of HOXD13 protein. B The effect of Hoxd13 mutation on osteoclast differentiation analyzed using TRAP staining. ***p < 0.001. C The effect of Hoxd13 mutation on the mRNA expression of osteoclast-associated genes. **p < 0.01; ***p < 0.001. D The effect of Hoxd13 mutation on the expression of osteoclast-associated proteins. *p < 0.05; **p < 0.01. All experiments were repeated at least three times.