Fig. 2: RNA-seq analyses of the effect of α-ketoglutarate and anti-PD-1 treatment on the gene expression profile. | Cell Death & Disease

Fig. 2: RNA-seq analyses of the effect of α-ketoglutarate and anti-PD-1 treatment on the gene expression profile.

From: Supplementation with α-ketoglutarate improved the efficacy of anti-PD1 melanoma treatment through epigenetic modulation of PD-L1

Fig. 2: RNA-seq analyses of the effect of α-ketoglutarate and anti-PD-1 treatment on the gene expression profile.

A Representative differential gene clustering map. B Enrichment map of GSEA signaling pathway enriched in α-KG + anti-PD1 group. C–F RNA-sequencing results were analyzed by the ImmuneCellAI-mouse method to characterize the immune microenvironment of melanoma tissue. The relative proportions of T cells (C, left panel), naive CD8+ T cells (C, right panel), CD8+ T cells (D, left panel), cytotoxic CD8+ T cells (D, right panel), central memory CD8+ T cells (E, left panel), effector memory CD8+ T cells (E, right panel) and M2 macrophages (F, left panel) and immune infiltration scores (F, right panel) in the tumor microenvironment. G, H Correlations of TET3 gene expression with the proportion of central memory CD8+ T cells (G, left panel), effector memory CD8+ T cells (G, right panel), cytotoxic CD8+ T cells (H, left panel) or immune infiltration scores (H, right panel). Multiple experimental data (n = 4) are counted and presented according to the statistical methods, and an asterisk (*) indicates the degree of significant difference.

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