Fig. 5: α-ketoglutarate upregulated the expression of TET2/3 and IFNγ-induced STAT1/3 and PD-L1 in melanoma cells. | Cell Death & Disease

Fig. 5: α-ketoglutarate upregulated the expression of TET2/3 and IFNγ-induced STAT1/3 and PD-L1 in melanoma cells.

From: Supplementation with α-ketoglutarate improved the efficacy of anti-PD1 melanoma treatment through epigenetic modulation of PD-L1

Fig. 5: α-ketoglutarate upregulated the expression of TET2/3 and IFNγ-induced STAT1/3 and PD-L1 in melanoma cells.

A, B Cell lysates were extracted from B16F10 A and Sk-Mel-28 B cells, and immunoblotting was then performed. C, D Extraction of mRNA and protein from treated melanoma cells, and qRT–PCR C and immunoblotting D were then performed. E, F Extraction of mRNA and protein from treated B16F10 tumor-bearing mouse tissues, and qRT-PCR E and immunoblotting F were then performed. G Detection of the expression of p-STAT1/3 and PD-L1 in B16F10 tumor-bearing mouse tissues by TSA-fluorescent multiplex immunofluorescence staining after the indicated treatments. Multiple experimental data (n = 3) are counted and presented according to the statistical methods, and an asterisk (*) indicates the degree of significant difference.

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