Fig. 2: USP22 increases ZEB1-induced VEGFA transcription in HCC cells.
From: USP22 upregulates ZEB1-mediated VEGFA transcription in hepatocellular carcinoma
A–C The effects of USP22 on protein expression of VEGFA in HCCLM3 or Huh7 cells with GAPDH as the reference. D, E Evaluation of mRNA expression of VEGFA in USP22 knockdown PLC/PRF/5 or Huh7 cells. F Evaluation of mRNA expression of VEGFA in USP22 overexpressed PLC/PRF/5 or Huh7 cells. G USP22 enhances VEGFA transcription. HEK293 cells were transfected with PGL3-Basic or PGL3-VEGFA to evaluate the effect of USP22 on the transcription of VEGFA. Cells were lysed and assayed using the dual-luciferase reporter assay system. H USP22 and ZEB1 co-upregulate VEGFA transcription. HEK293 cells were transfected with PGL3-VEGFA together with the indicated expression plasmids. Cells were lysed and assayed using the dual-luciferase reporter assay system. I USP22 lost its regulatory effect on VEGFA protein expression when ZEB1 was knocked down. FLAG-USP22 or Vector was transfected with or without ZEB1 siRNA into Huh7 cells. J, K Evaluation of mRNA expression of three ZEB1 target gene in USP22 knockdown HCCLM3 or PLC/PRF/5 cells.
