Fig. 3: PTPN14 promotes cell proliferation, migration, and invasion in vitro.
From: PTPN14 promotes gastric cancer progression by PI3KA/AKT/mTOR pathway
A–C Western blot verified the overexpression or knockdown efficacy of PTPN14. In AGS cells, PTPN14 expression was decreased by shRNA (A). In BGC-823 cells, LV-PTPN14 stably increased the expression of PTPN14 (B). In MKN-45 cells, LV-PTPN14 stably increased the expression of PTPN14 (C). D–F CCK-8 assays showed that PTPN14 knockdown inhibited cell proliferation in AGS cells (D), while overexpression of PTPN14 promoted cell proliferation in MKN-45 cells (E) and BGC-823 cells (F). **p < 0.01; cells overexpressing PTPN14 or knockdown PTPN14 were compared with control cells. G–I EDU assays showed that PTPN14 promoted cell proliferation. In AGS cells, the shPTPN14 group showed fewer EDU-positive cells than the control group (G). The LV-PTPN14 group showed more EDU-positive cells than the control group in MKN-45 cells (H) and BGC-823 cells (I). J–L Transwell assays indicated that PTPN14 promoted gastric cancer cell migration and invasion. In AGS cells, the shPTPN14 group showed fewer migrating or invading cells than the control group (J). The LV-PTPN14 group showed more migrating or invading cells than the control group in MKN-45 cells (K) and BGC-823 cells (L).
