Fig. 7: The effects of METTL3 knockdown were restored by the simultaneous upregulation of DDX23. | Cell Death & Disease

Fig. 7: The effects of METTL3 knockdown were restored by the simultaneous upregulation of DDX23.

From: METTL3 enhances pancreatic ductal adenocarcinoma progression and gemcitabine resistance through modifying DDX23 mRNA N6 adenosine methylation

Fig. 7

A The knockdown of METTL3 and overexpression of DDX23 were confirmed at the translational level. B The suppressed OD values of the two cell lines by METTL3 knockdown were restored by the simultaneous overexpression of DDX23. C, D The suppressed cell proliferation resulting from METTL3 silence was partially restored by DDX23 upregulation. E The elevated cell apoptosis resulting from METTL3 knockdown was partially reversed by DDX23 demonstrated by the TUNEL assay. F The knockdown of METTL3 led to suppressed PI3K and Akt phosphorylation as well as cell cycle-related proteins, which were elevated by DDX23 overexpression. G, H DDX23 overexpression enhanced GEM resistance of cells with METTL3 knockdown, demonstrated by CCK8 assay (G) and TUNEL assay (H). **P < 0.01, ***P < 0.001.

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