Fig. 3: ETV7 reduces NF-κB signaling by repressing TNFRSF1A.
From: ETV7 reduces inflammatory responses in breast cancer cells by repressing the TNFR1/NF-κB axis
A Gene reporter assays in MCF7 and T47D cells over-expressing ETV7 or its empty counterpart transiently transfected with the pGL3-NF-κB reporter plasmid. Data is normalized using the Renilla reniformis luciferase reporter vector pRL-SV40 and shown as fold of induction relative to the Empty control. B Gene reporter assays in MCF7 (on the left) and T47D (on the right) cells over-expressing ETV7 or its empty counterpart transfected with the pGL3-NF-κB reporter plasmid and stimulated with TNF-α (10 ng/ml for MCF7 and 15 ng/ml for T47D), IL-6 (20 ng/ml) or combination of both for 4 h. Data are normalized using the Renilla reniformis luciferase reporter vector pRL-SV40 and shown as fold of induction relative to the Empty control. C–F RT-qPCR analysis of known NF-κB target genes: A20 (C), IL-8 (D), IL-6 (E), and TNF-α (F) in MCF7 ETV7 or Empty cells treated with TNF-α (10 ng/ml) for 4 h. Bars represent the averages and standard deviations of at least three biological replicates. G RT-qPCR analysis of the normalized expression of NF-κB target genes in MCF7 parental cells transfected with ETV7 targeting siRNA #1 and siRNA #2 or the scrambled control. Whole panel: Bars represent the averages and standard deviations of at least three biological replicates. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001, n.s. not significant.
