Fig. 4: Transcriptional dependence on SWI/SNF subcomplexes. | Cell Death & Disease

Fig. 4: Transcriptional dependence on SWI/SNF subcomplexes.

From: GEMC1 and MCIDAS interactions with SWI/SNF complexes regulate the multiciliated cell-specific transcriptional program

Fig. 4

A Validation of AD-293 ARID1A knock-out (KO) cells. Western blots for ARID1A and BRD9 are shown. Actin and Ponceau staining serve as loading and transfer controls. B Quantitative real-time PCR (qRT-PCR) analysis of CCNO and FOXJ1 expression in AD-293 cells following transfection with the indicated genes. WT vs ARID1A-KO p-values were 0.0287* and 0.045* for GEMC1 and 0.026* and 0.007** for MCIDAS. Transfected genes are color coded in the key to the right. C. qRT-PCR analysis of HSPAL1 and CCDC96 in parental or ARID1A-KO AD-293 cells. Statistical analysis as in B revealed no significant differences. D qRT-PCR analysis of HSPAL1 and CCDC96 in parental or ARID1A-KO AD-293 cells treated with 5 μM of I-BRD9. WT vs I-BRD9 p-values were 0.011* (HSPA1L), 0.006**(CCDC96), and E 0.0006***(FOXJ1) for MCIDAS. Color coding as shown in panel B. F qRT-PCR analysis of HSPAL1 and CCDC96 expression in AD-293 cells following transfection with the indicated genes. Transfected genes are color coded in the key to the right. For B–F, results from three independent experiments are shown with the bars indicating the mean and the standard deviation shown. For statistical analysis a paired t-test was used, ***p ≤ 0.001, **p ≤ 0.01, and *p ≤ 0.05.

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