Fig. 6: Proximal labeling study in HEK293 cells with rat IgG2B transmembrane form as a bait. | Cell Death & Disease

Fig. 6: Proximal labeling study in HEK293 cells with rat IgG2B transmembrane form as a bait.

From: Astrocytes express aberrant immunoglobulins as putative gatekeeper of astrocytes to neuronal progenitor conversion

Fig. 6

A Detection of biotinylated proteins (green, Streptavidin Alexa Fluor 488 conjugate) and rat IgG2B (red, anti-Flag) in HEK293 cells after overexpression of IgG2B in fusion with BirA*Flag. IgG2B was overexpressed with a rat or a human signal peptide. Nucleis were stained with DAPI (blue). A control consisting of the overexpression of the transmembrane domain itself fused to a rat or a human signal peptide has been included in the experiment. The inset represents a zoom of the results observed after detection of IgG2B with anti-Flag. B Systemic biology representative of proximal interactome of rat IgG2B transmembrane form.

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